Agastya, I Made Indra and Ameliawati, Presti and Fikrinda, Wahyu
(2018)
Eksplorasi dan identifikasi Jamur Patogen Serangga di Rhizosfer
Lahan Kering Kabupaten Malang.
Politeknik Negeri Lampung.
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Abstract
This research was conducted by exploring insect pathogen in rhizosphere of dry field of sugar
cane of Malang Regency. The purpose of this research is to study the diversity of isolate insect
pathogen in rhizosphere of sugar cane plant in dry land of Malang Regency. Exploration
activities of insect pathogens are carried out by isolating insect pathogens in dry land of
sugarcane plant rhizosphere. Rizosphere soil sampling method using composite sampling
technique is by taking diagonal soil samples at five points as deep as 5-10 cm, as much as 200
g at each sample point and isolation of insect pathogen type fungus using baiting method. The
soil samples from the field were placed evenly over the petri dish. The soil above the petri dish
is moistened and then the larvae of T miltor are placed for incubation for 48 hours. After 48
hours of dead larvae there will be a sign of an insect pathogenic fungus in the form of white
hyphae on the surface of the T milter body. The marked larvae were then cultured on PDA
media to be purified and identified morphologically, by looking at the color of hyphae.
Observation miscroscopically using Burnet and Hunter determinations. Exploration and
identification results found four specimens of fungi with Aspergilus sp species, Trichoderma
sp, Beuveria sp, and Metharizium sp
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Eksplorasi dan identifikasi Jamur Patogen Serangga di Rhizosfer
Lahan Kering Kabupaten Malang. (deposited 08 Oct 2020 04:52)
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